Biology is the antithesis of engineering: soft, free-form shapes; interconnected, active matter; persistent reorganization and adaptation. Selectively omitting these traits in favor of simplicity forsakes clinical relevancy - only in the laboratory does life grow on a plastic surface.
Aurita’s technologies forego the cellular monolayer, and instead emphasize the role of the microenvironment and the interactions within. These technologies culminate in iVITA™, or in Vitro Immuno Therapy Assays, which comprise all of Aurita’s technologies and protocols.
Darcy™The Darcy™ perfusion-enabled 3D culture ecosystem revolutionizes the study of life in miniature by leveraging perfusion, or the flow of culture media through LLS™, in vitro. This breakthrough emulates the physiological transport dynamics which occur in capillary beds, resulting in prolonged sample viability and the retention of microtissue function.
Our latest design, the 24-Well Darcy™ imaging insert, integrates an optically transparent permeable membrane to achieve both the benefits of perfusion culture and the analysis of in situ imaging.
LLS™ microgels can be conjugated with most biopolymers including type I collagen, fibronectin, and laminin to support cell adherence, migration, and signaling. Channels and crevices throughout the LLS™ particle bed mimic a capillary network giving cells a chance to migrate, receive mechanical cues, and follow cytokine gradients.
Left: Murine glioma cells cultured in collagen-1 bioconjugated LLS™ grow and wrap around LLS™ particles to form a network structure. LLS™ particles are totally transparent, supporting in-situ imaging without obscuring the cells of interest.
Above: Murine glioma cells cultured in collagen-1 bioconjugated LLS™ grow and wrap around LLS™ particles to form a network structure. LLS™ particles are totally transparent, supporting in-situ imaging without obscuring the cells of interest.
