A 3D BioScience Company

Biology is the antithesis of engineering: soft, free-form shapes; interconnected, active matter; persistent reorganization and adaptation. Selectively omitting these traits in favor of simplicity forsakes clinical relevancy - only in the laboratory does life grow on a plastic surface.

Aurita’s technologies forego the cellular monolayer, and instead emphasize the role of the microenvironment and the interactions within. These technologies culminate in iVITA™, or in Vitro Immuno Therapy Assays, which comprise all of Aurita’s technologies and protocols.

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Featured Solutions

Integrating Perfusion in 3D Culture

Darcy™The Darcy perfusion-enabled 3D culture ecosystem revolutionizes the study of life in miniature by leveraging perfusion, or the flow of culture media through LLS, in vitro. This breakthrough emulates the physiological transport dynamics which occur in capillary beds, resulting in prolonged sample viability and the retention of microtissue function.

Our latest design, the 24-Well Darcy imaging insert, integrates an optically transparent permeable membrane to achieve both the benefits of perfusion culture and the analysis of in situ imaging.

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Metastasis: Glioblastoma cells crawl across COL-1 conjugated LLS™ microgels.

Image series captured in situ over 5 days using a Nikon A1R confocal microscope.
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3D BioAssays

The Study of Life in Miniature

Functional

Tune the stiffness, pore size, fluid velocity, and functional coating of LLS™ to the needs of any experiment. Coating LLS™ particles with extracellular matrices expands control of the microenvironment inducing functions such as motility, adhesion, spheroid formation, and growth.

Validated

Perfusion-enabled 3D culture facilitates a comprehensive library of applications including immunology, pathophysiology, metabolomics, and personalized medicine, each validated using a wide range of tissues and cell types which retain their native functionality and growth forms.

Accessible

Aurita’s 3D Bioassays are developed around ubiquitous laboratory consumables, tools, and equipment, streamlining their integration into established workflows. Standardized SBS microplate formats ensure that Darcy perfusion plates™ are compatible with all automation and liquid handling systems.

Biomimicking

Liquid-Like Solids (LLS™)  support and preserve delicate tissue structures and form an open matrix of fluid channels which mimic microvasculature. Darcy Plates™ facilitate perfusion of culture media through this porous medium, emulating capillary bed transport dynamics and prolong both sample viability and function.

Complex growth in 3D with ECM Bioconjugated LLS™

LLS™ microgels can be conjugated with most biopolymers including type I collagen, fibronectin, and laminin to support cell adherence, migration, and signaling. Channels and crevices throughout the LLS™ particle bed mimic a capillary network giving cells a chance to migrate, receive mechanical cues, and follow cytokine gradients.

Left: Murine glioma cells cultured in collagen-1 bioconjugated LLS™ grow and wrap around LLS™ particles to form a network structure. LLS™ particles are totally transparent, supporting in-situ imaging without obscuring the cells of interest.

Above: Murine glioma cells cultured in collagen-1 bioconjugated LLS™ grow and wrap around LLS™ particles to form a network structure. LLS™ particles are totally transparent, supporting in-situ imaging without obscuring the cells of interest.

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